How to use application variables in UnitTest asp.net

I have a method that uses Application variables to get information from an external file. Since Application variables are not used in unit tests, is there a way I can get the Application variables values from my Global.asax file and be able to use them in the test?

This is my test method:

[TestMethod]
public void TestGetCompanyList()
    {
        var accountController = new AccountServiceController();
        CompanyInput cInput = new CompanyInput();
        cInput.IssuerName = "Other";
        cInput.Ticker = "BR";
        var result 
          = accountController.GetCompanyList(cInput) as IEnumerable<CompanyListResult>;
        Assert.IsNotNull(result);
    }